Substrate induced radical formation in 4 - hydroxybutyryl - CoA dehydratase 1 from Clostridium aminobutyricum
نویسندگان
چکیده
3 Jin Zhang*, Peter Friedrich*, Antonio J. Pierik, Berta M. Martins, Wolfgang Buckel 4 5 Laboratorium für Mikrobiologie, Fachbereich Biologie and Synmikro, Philipps-Universität, 35032 6 Marburg, Germany; Max-Plank-Institut für terrestrische Mikrobiologie, 35043 Marburg; Institut 7 für Zytobiologie, Philipps-Universität, 35032 Marburg, Germany; Present address: Fachbereich 8 Chemie, Universität Kaiserslautern, 67663 Kaiserslautern, Germany; Institut für Biologie, 9 Strukturbiologie/Biochemie, Humboldt-Universität zu Berlin, 10099 Berlin, Germany. 10 11 Dedicated to Dr. Peter Willadsen (Brisbane, Australia) 12 * JZ and PF contributed equally to this work and are considered both as first authors. 13 Running head: Mechanism of 4-hydroxybutyryl-CoA dehydratase 14 Correspondence to: W. Buckel, Laboratorium für Mikrobiologie, Fachbereich Biologie, Karl-von15 Frisch-Straße 8, 35032 Marburg. Tel +49 6421 28 22088, Fax +49 6421 28 28979, Email: 16 [email protected] 17 18
منابع مشابه
Substrate-induced radical formation in 4-hydroxybutyryl coenzyme A dehydratase from Clostridium aminobutyricum.
4-Hydroxybutyryl-coenzyme A (CoA) dehydratase (4HBD) from Clostridium aminobutyricum catalyzes the reversible dehydration of 4-hydroxybutyryl-CoA to crotonyl-CoA and the irreversible isomerization of vinylacetyl-CoA to crotonyl-CoA. 4HBD is an oxygen-sensitive homotetrameric enzyme with one [4Fe-4S](2+) cluster and one flavin adenine dinucleotide (FAD) in each subunit. Upon the addition of crot...
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